Reader in Biochemistry in the School of Biochemistry, University of Bristol.
Jan's research focuses on the development of in vitro systems to generate human erythroid cells from different stem cell sources, including adult, cord and iPSCs, and the molecular analysis of these cells.
Jan Frayne obtained her first degree from Nottingham University and PhD from Bristol University before commencing research in developmental biology, specifically sperm function. She moved to the Department of Biochemistry at the University of Bristol on a post-doctoral position to develop skills in molecular biology, obtaining a lectureship here in 2000.
Although still maintaining an active interest in this field, she more recently moved into the field of erythropoiesis, in particular the molecular analysis of erythropoiesis, and the development of innovative proteomic approaches to qualitatively and quantitatively compare the differential proteome of erythroblasts from the different types of stem cells.
Many of her studies focus on transcription factors and in collaboration with Prof Anstee of NHSBT, was the first to identify and report a mutation in KLF1 that results in a severe human disease phenotype, and to demonstrate how this and other mutations affect KLF1 function.
She has published over 40 peer-reviewed papers.
Within the “Biology Workstream” of the NOVOSANG project her research aims to use novel proteomic approaches to compare the proteome of the erythroid cells generated from pluripotent and adult stem cells, and identify regulatory and other proteins that differ in expression which can be manipulated using forwarded programming approaches.
Recent publications of relevance include comparison of the proteome of iPSC and adult derived erythroid cells using multiplex TMT labeling and NanoLC-MS/MS, and forward programming of stem cell derived erythroid cells to switch the globin expression from an embryonic or fetal to an adult phenotype.